Coding
Part:BBa_K1530000:Design
Designed by: Yao Chin Liu Group: iGEM14_Auckland_New_Zealand (2014-12-17)
Homo sapiens (Human) Epithelial Cadherin Extracellular Domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 129
Illegal AgeI site found at 183
Illegal AgeI site found at 519
Illegal AgeI site found at 543
Illegal AgeI site found at 1167
Illegal AgeI site found at 1278 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Optimized the codon sequence for use in E. coli.
Have included a BamH1 restriction site for ease of attachment to other parts.
Source
This part is taken from the Uniprot database (http://www.uniprot.org/uniprot/P12830). Only the extracellular portion of the whole Human E-cadherin protein was used.