Coding

Part:BBa_K1530000:Design

Designed by: Yao Chin Liu   Group: iGEM14_Auckland_New_Zealand   (2014-12-17)

Homo sapiens (Human) Epithelial Cadherin Extracellular Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 129
    Illegal AgeI site found at 183
    Illegal AgeI site found at 519
    Illegal AgeI site found at 543
    Illegal AgeI site found at 1167
    Illegal AgeI site found at 1278
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized the codon sequence for use in E. coli.

Have included a BamH1 restriction site for ease of attachment to other parts.

Source

This part is taken from the Uniprot database (http://www.uniprot.org/uniprot/P12830). Only the extracellular portion of the whole Human E-cadherin protein was used.

References